Skin Graft Mincing and 100-fold Expansion in the or - an Alternative to in Vitro Cultures in the Treatment of Large

نویسنده

  • BURNS
چکیده

Introduction: Split-thickness skin graft (STSG) is the gold standard for treatment of major skin loss from major trauma or burn injuries. However, this technique is limited by the donor site availability in large burn injuries. Several techniques have been developed in the past to overcome this limitation but have been unable to achieve clinical relevance. With skin mincing technique, we have already successfully demonstrated an expansion ratio of 1:100 and wound healing comparable to STSG [1].

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

BIO treatment enhances rat marrow-derived mesenchymal stem cell in vitro proliferation and viability

Introduction: Previous investigations have indicated that the presence of BIO (6-Bromoindirubin-3-Oxime) in medium of some cell culture enhances the cell proliferation and viability. The aim of the present study was to investigate the BIO effects on in vitro expansion of rat marrow-derived mesenchymal stem cells (MSCs) culture. Methods: In the present experimental study, bone marrow cells from ...

متن کامل

Effects of different culture media on optimization of primary neuronal cell culture for in vitro models assay

Background: In vitro model studies are becoming increasingly popular for experimental research designs. They include isolation and expansion of cells of a particular tissue, such as the nervous tissue which contributes to understanding the underlying mechanisms in many pathologies. It enables  the scrutinization of intracellular signaling pathways responsible for cell death. OBJECTIVES: In the ...

متن کامل

Ex vivo Expansion and Differentiation of Mesenchymal Stem Cells from Goat Bone Marrow

Objective(s) Mesenchymal stem cells (MSCs) from large animals as goat which is genetically more closely related to human have rarely been gained attentions. The present study tried to isolate and characterize MSCs from goat bone marrow. Materials and Methods Fibroblastic cells appeared in goat marrow cell culture were expanded through several subcultures. Passaged-3 cells were then different...

متن کامل

Repair of a Scalp Defect with Punctate Skull Fenestration and Skin Grafting

Reconstructing scalp defects can be challenging for a plastic surgeon. For this purpose, different procedures such as primary repair and coverage with skin grafts or flaps can be considered. Sometimes, the external table of the skull can be removed completely to the diploic space. At the same time or after the formation of granulation tissue, the diploic space can be covered with split-thicknes...

متن کامل

Evaluating the effect of medical honey and stapler on skin graft fixation in burn wounds: A Randomized clinical Trial

Background: One of the uses of honey is related to wound healing. A stapler normally connects the skin graft to the underlying tissue. Due to its high cost, lack of production in the country, and causing physical damage to the tissue, an alternative option is needed. Objective: This study aimed to determine the effect of using medical honey compared to staplers in attaching skin grafts to burn...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2015